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Published Pages | hb-superuser | Merge multiple BED files into single categorical track

Merge multiple BED files into single categorical track

This tool allows combining elements from multiple datasets into a single track, denoted with a category that reflects their source.

In this example, we combine exon, intron and intergenic regions of the Human genome (hg19) into a single track. 

  • The tool operates on tracks from history, so Exons and Introns are exported from the HyperBrowser repository to the history (Step 1 & 2)
  • Intergenic regions are not in the hierarchy, but are made from the whole genome (Step 3) subtracted by Exons and Introns (Step 4 & 5)
  • The three datasets were then converted to category.bed using the "Merge multiple BED files into single categorical track" tool (Step 6)
  • The track is sorted in order to see that the whole genome now is covered with exons, introns or intergenetic regions (Step 7)
  • In order to use the category track further, we cut it to just include 4 columns, as intergenic regions have no strand column defined. (Step 8)
  • An example analysis of a category track is run which shows that exons cover 3.4% of the genome. (For the analysis to be run, the data type of the history element must be changed from 'interval' to 'category.bed' prior to analysis. Note also that the sum of the coverage of all categories exceed 1, as some exons are in introns of other transcripts.) (Step 9)

You may import the history by clicking the "import history" button below. You will see an overview of the files and parameter settings in the tools.